• Narra venkatesh B.Pharm Research Scholars, A M Reddy Memorial College of Pharmacy, Petlurivaripalem Narasaraopeta, Guntur Dist, Andhra pradesh.
  • Kopuri Gayathri Devi B.Pharm Research Scholars, A M Reddy Memorial College of Pharmacy, Petlurivaripalem Narasaraopeta, Guntur Dist, Andhra pradesh.
  • Gunji Venkateswarlu Assoc. Prof, Department of Pharmacognosy, A M Reddy Memorial College of Pharmacy, Petlurivaripalem Narasaraopeta, Guntur Dist, Andhra pradesh
  • Akula Murali Sri Sudhakar Prof.Department of Pharmaceutics, A M Reddy Memorial College of Pharmacy, Petlurivaripalem Narasaraopeta, Guntur Dist, Andhra pradesh.


Objective: The aim of the study is to evaluate the effect of the Hydroalcoholic extract of Moringa oleifera leaves on fertility hormone and semen quality of male albino rats. Materials and method: The Hydroalcoholic extract of Moringa oleiferaseed at doses of 100, 200 and 400 mg/kg were administrated for 30 days. The effect of the extract on body weight and sexual organs weights (testes and epididymis) were determined. The fertility hormone and semen characteristic was studied. Resultsand and discussion: Oral administration of Hydro alcoholic extract at doses of 100, 200 and 400 mg/kg were significantly increased body weight and sexual organ weight. Also significantly increased serum Testosterone, Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH) compared to the control group, in addition, significantly increased semen characteristic in experimental animals study. Conclusion :The results of the present study demonstrate the effectiveness of Moringa oleiferaseed extract on fertility hormones stimulator and improvement of semen characteristic which justify the traditional use of the plant as aphrodisiac and for management of male certain sexual disorders

Keywords: Albino rats, fertility hormone, Moringa oleifera, body weight


Download data is not yet available.


1. Bhatia, D. K. Sharma, A. K. Pathania P. C. and Khanduri N. C. (2010). Antifertility effects of crude different of Adiantumlunulatum Burm. on Reproductive Organs of male albino rats. Biological Forum-An International Journal, 2(2): 88-93.
2. Yakubu, M. T.; Akanji, M. A. and Oladiji, A. T. (2007). Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. PHCOG Rev., 1(1): 49-52.
3. Sumalatha, K.; Saravana, K. A. and Mohana, L. S. (2010). Review of natural aphrodisiac potentials to treat sexual dysfunction. Int J Pharm Ther., 1: 10-18.
4. Mughal, M. H.; Ali, G.; Srivastava, P. S. and Iqbal, M. (1999). Improvement of drumstick (Moringapterygosperma Gaertn)-a unique source of food and medicine through tissue culture. Hamdard Med., 42: 37–42.
5. Lalas, S. and Tsaknis, J. (2002). Extraction and identification of natural antioxidants from the seeds of Moringa oleifera tree variety of Malavi. J. Am. Oil. Chem. Soc., 79: 677–683.
6. Mishra, G.; Singh, P. and Verma, R. (2011) “Traditional uses, phytochemistry and pharmacological properties of Moringa oleifera plant: an overview,” Der Pharmacia Lettre, 3(2): 141–164.
7. Subadra, S.; Monica, J. and Dhabhai, D. (1997). Retention and storage stability of beta- carotene in dehydrated M. oleifera. Inter J Food Science and Nutri, 48: 373 – 379.
8. Faye, B.; Bucheton, B. and Banuls, A. L. (2011). Prevalence of leishmaniainfantum in a rural area of Senegal: analysis of risk factors involved in transmission to humans. J. Trans. R. Sco. Trop. Med Hyg., 105: 333 – 340.
9. Dorga, P.; Singh, D. and Tandon, S. (1975). Vitamin content in Moringa. J. Current Sci., 44: 30 – 31.
10. Shukla S., Mathur R., Prakash AO. Antifertility profile of the aqueous extract of Moringa oleifera roots. J-Ethnopharmacol. 1988; 22(1): 51-56.
11. Mori, S.; Cameldi, I. and Pardini, A. (2009). Moringa oleifera: a promising multipurpose tree for tropical and subtropical areas. Associazione Scienze Agrarie Tropicali, 1: 1-2.
12. Ashfaq, M., Basra, S. and Ashfaq, U. (2011). "MORINGA" A Miracle Plant of Agro Forestry and Southern Punjab, PAKISTAN. World Environment Day, 16th: 41-54.
13. Tesfay, S., I. Bertling, A. Odindo, T. Workneh, N. Mathaba, (2011). Levels of anti- oxidants in different parts of moringa (Moringa oleifera) seedling. African Journal of Agricultural Research, 6(22): 5123-5132.
14. Sabu, M.C. and Subburajub, T. (2002). Effect of Cassia auriculata Linn. on serum glucose level, glucose utilization by isolated rat hemidiaphragm. J. of Ethnopharmacology, 80(2- 3): 203-6.
15. Thakur, M. and Dixit, V. K. (2007). Aphrodisiac activity of Dactylorhizahatagirea (D. Don) Soo in male albino rats. Evid. Based Compl. Alternate Med., 4: 29-31.
16. Amini, A. and Kamkar, F. (2005). The effects of gossypol on spermatogenesis in NMRI mice. Iranian J. Sciand Technol Trans., 29: 123-133.
17. Prasad, M. R., Chinoy, N. J. and Kadam, K. M. (1972). Changes in succinic dehydrogenase levels in the rat epididymis under normal and physiologic conditions. Fertil Steril, 23: 186-190.
18. Raji. Y.; Udoh, U. S.; Mewoyeka, O. O.; Onoye, F. C. and Bolarinwa, A. F. (2003). Imolication of reproductive endocrine malfunction in male fertility efficacy of Azadirachtaindicaextract in rats. Afr. J. Med. Sci., 32: 159-165.
19. Kisa, U.; Basar, M. M.; Ferhat, M.; Yilmaz, E.; Basar, H. and Caglayan, O. (2004). Testicular tissue nitric oxide and thiobarbituric acid reactive substance levels: evaluation with respect to the pathogenesis of varicocele. Urol. Res., 32: 196-199.
20. Lilibeth, A. C. and Glorina, L. P. (2010). Effects of Moringa oleifera Lam. (Moringaceae) on the reproduction of male mice (Mus musculus). J. Med. Plant. Res., 4: 1115-1121.
21. Makkar, H. P. and Becker, K. (1996). Nutritional value and antinutritional components of whole and HYDROALCOHOLIC extraction Moringa oleiferaleaves. J. Animal Feed Sci. Technol., 63: 311-322.
22. Ram J. (1994). Moringaa highly nutrition vegetable tree. Trides Technical Bulletin. pp. 2.
23. Dorga, P.; Singh, D. and Tandon, S. (1975). Vitamin content in Moringa. J. Current Sci., 44: 30 – 31.
24. Akunna, G. G.; Ogunmodede, O. S.; Saalu, C. L.; Ogunlade, B.; Bello, A. J. and Salawu, E. O. (2012). Ameliorative Effect of Moringa oleifera (drumstick) Leaf Extracts on Chromium-Induced Testicular Toxicity in Rat Testes World Journal of Life Sciences and Medical Research, 2: 20-26.
25. Sonmez, M.; Turk. G. and Yuce, A. (2005). The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology, 63: 2063-2072.
26. Yousef, M. I.; Esmail, A. M. and Baghdadi, H. H. (2004). Effect of isoflavones on reproductive performance, testosterone levels, lipid peroxidation, and seminal plasma biochemistry of male rabbits. J. Environ Sci. Health B., 39: 819-833
27. El-Missiry, M. A. (1999). Enhanced testicular antioxidant system by ascorbic acid in alloxn diabetic rats. Comparative Biochemistry and Physiology, 124: 233-237.
28. Ghosh, D., Das, U. B., Misro, M. (2002). Protective role of alpha-tochopherol- succinate in cyclophosphamide induced testicular gametogenic steroidogenic disorders: a correlative approach to oxidative stress. Free Radical Research, 36: 1199-1208.
29. Kujo, S. (2004). Vitamin C: basic metabolism and its function as an index of oxidative stress. Current Medicinal Chemistry, 11: 1041-1064.
30. Dawson, E. B., Harris, W. A. and Powell, S. A. (1990). Relationship between ascorbic acid and male fertility. World Review of Nutrition Dietetics, 62: 1-26.
31. Luck, M. R., Jeyaseelan, I., Scholes, R. A. (1995). Ascorbic acid and fertility. Biology of Reproduction, 52: 262-266.
32. Salem, M. H., Kamel, K. I., Yousef, M. I., Hassan, G. A., EL- Nouty, F. D. (2001). Protective role of ascorbic acid to enhance semen quality of rabbits treated with sublethal doses of aflatoxin B1. Toxicology, 162: 209-218.
33. Mukhallad, A.M., Mohamad, M.J.M. and Dradka, H. (2009). Effects of black seeds (Nigella sativa) on spermatogenesis and fertility of male rats. Res J. Med Med Sci., 4: 386-390.
34. Aversa, A. and Fabbri, A. (2001). New oral agents for erectile dysfunction: what is changing in our practice? Asian J. Androl., 3: 175-179.
35. Morels, A. (1996). Androgen supplementation in practice: the treatment of erectile dysfunction associated with hypotestosteronemia. In BJ Oddens, Vermeulen A editor. Androgens and aging male. London: Parthenon Publishing Group, 233-245.
36. Watcho, P.; Donfack, M. M.; Zelefack, F.; Nguelefack, T. B.; Wansi, S.; Ngoula, F. and Kamanyi, A. (2005). Effects of the hexane extract of Mondiawhiteion the reproductive organ of male rat. Afr. J. Trad. CAM., 2(3): 302-311.
37. El-Tahomi M. M., El-Nattat W. S. and El-Kady R. I. (2010). The beneficial effects of Nigella sativa, Raphanus sativus and Eruca sativa seed cakes to improve male rabbit fertility, immunity and production. J. Am. Sci., 6: 1247-1255.
38. Gauthaman, K. and Adaikan, P. G. (2008). The hormonal effect of Tribulus Terrestrisand role its role in the management of erectile dysfunction- an evaluation using primates rabbit and rats. Phytomedicine, 15(1): 44-54.
39. Singh. J.; O’Neill, C. and Handelsman, D.J. (1995). Induction of spermatogenesis byandrogens in gonadotropin-deficient (hpg) mice. Endocrinology, 136: 5311-5321.
40. O’Donnel, L.; Mc Lachlan, R.I.; Wreford, N.G. and Robertson, D.M, (1994). Testosterone promotes the conversion of round spermatids between stages vii and viii of the rat spermatogenic cycle. Endocrinology, 135: 2608-614.
320 Views | 157 Downloads
How to Cite
venkatesh, N., K. G. Devi, G. Venkateswarlu, and A. M. S. Sudhakar. “EFFECT OF HYDROALCOHOLIC EXTRACT OF MORINGA OLEIFERA LEAVES ON FERTILITY HORMONE AND SPERM QUALITY OF MALE ALBINO RATS”. World Journal of Current Medical and Pharmaceutical Research, Vol. 1, no. 3, June 2019, pp. 83-87, https://www.wjcmpr.com/index.php/journal/article/view/18.
Research Articles