Development and validation of new analytical method for the simultaneous estimation of daunorubicin and cytarabine in bulk and pharmaceutical dosage form

  • Kankipati Benjimen Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.
    mlcollegeofpharmacy@gmail.com
  • K. Thejomoorthy Head, Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.
  • P.Sreenivasa Prasanna Principal, M.L.College of Pharmacy, S.Konda-523101.

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Daunorubicin and Cytarabine inhalation dosage form. Chromatogram was run through BDS C18 150 x 4.6 mm, 5m. Mobile phase containing 0.1% OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 240nm. Retention time of Daunorubicin and Cytarabine were found to be 2.245 min and 2.813. %RSD of the Daunorubicin and Cytarabine were and found to be 0.6and 0.3 respectively. %Recovery was obtained as 99.39% and 99.26% for Daunorubicin and Cytarabine respectively. LOD, LOQ values obtained from regression equations of Daunorubicin and Cytarabine were 0.03, 0.10 and 0.31, 0.94 respectively. Regression equation of Daunorubicin is y = 2677x + 703.5, y = 2524x + 104.7 of Cytarabine.Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.  

Keywords: Daunorubicin and Cytarabine, RP-HPLC

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Author Biographies

Kankipati Benjimen, Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.

Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.

K. Thejomoorthy, Head, Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.

Head, Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.

P.Sreenivasa Prasanna, Principal, M.L.College of Pharmacy, S.Konda-523101.

Principal, M.L.College of Pharmacy, S.Konda-523101.

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Published
07-05-2021
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How to Cite
Kankipati Benjimen, K. Thejomoorthy, and P.Sreenivasa Prasanna. “Development and Validation of New Analytical Method for the Simultaneous Estimation of Daunorubicin and Cytarabine in Bulk and Pharmaceutical Dosage Form”. World Journal of Current Medical and Pharmaceutical Research, Vol. 3, no. 3, May 2021, pp. 32-39, doi:10.37022/wjcmpr.vi.171.
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Research Articles