PHYTOCHEMICAL AND IN-VITRO PHARMACOLOGICAL SCREENING OF OCIMUM KILIMANDSCHARICUM EXTRACT
Abstract
Ocimum kilimandscharicum is a short herb, native to India. In traditional medicine, it is used to treat various ailments including colds, coughs, abdominal pains, measles and diarrhea. Medicative plants, the “back bone” of ancient medication which implies over 3.3 billion folks within the less developed countries utilize medicative plants on an everyday basis. Ocimum kilimandscharicum Guerke (Syn. Ocimum camphora Guerke) belongs to family Lamiaceae. It is a native of Kenya and distributed in East Africa, India, Thailand, Uganda and Tanzania. It is extensively grown in the Tropics. In India it is cultivated on a small scale, especially in West Bengal, Assam, Tamil Nadu, Karnataka, Kerala and Dehradun. .The present study is aimed to evaluate in vitro anti-inflammatory, assessment was carried out on the basis of parameters such as heat induced protein denaturation, heat induced haemolysis, and Hypotonicity induced haemolysis. The standard drug was Diclofenac sodium. The findings of the present study showed that the 500 µg/ml of Ethanolic extraction of Ocimum kilimandscharicum ( EEOK) showed more percentage inhibition when compared to standard. On the basis of results, it can be concluded that Ethanolic extraction of Ocimum kilimandscharicum (EEOK) showed significant anti- inflammatory activity.
Downloads
References
2. Thaibinh ton thot. Herbal medicine. Ind. J. pharma. Edu, 1998; 32(2), 104-106.
3. Anonymous. Wealth of India: A dictionary of Indian raw materials and industrial products. New Delhi: CSIR, 1966; Vol-VII: 85.
4. Kashyap CP, Kaur R, Arya V, Kumar. Therapeutic Potency of Ocimum kilimandscharicum Guerke – A Review. Global Journal of Pharmacology, 2011; 5(3): 191-200.
5. Pullaiah T. Encyclopaedia of world medicinal plants. Regency, Vol-3. p. 1424.
6. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer, p. 445; 2007.
7. Lowman MS, Kelly JW. Camphor from Camphor Basil (Ocimum kilimandscbaricumGuerke). J Am Pharm Assoc., 1945; 34(6): 153-62.
8. Verma S, Kothiyal P. Pharmacological activities of different species of Tulsi. IJBPR. 2012; 1(1): 21-39.
9. Paton A. A Synopsis of Ocimum L. (Labiatae) in Africa. Kew Bulletin. 1992; 47(3): 403-35.
10. AnandAnkurKumar,ManindraMohan,S. ZafarHaider, Akash Sharma. Essential oil composition and antimicrobial activity of three Ocimum species from uttarakhand (india). International journal of pharmacy and pharmaceutical sciences, 2011; 3: 223-225.
11. Eliningaya J. Kweka1, Hassan M. Nkya, Lucile Lyaruu, Epiphania E. Kimaro, Beda J. Mwang’onde,Aneth M. Mahande. Efficacy of Ocimum kilimandscharicum plant extracts after four years of storage against Anopheles gambiaess. Journal of Cell and Animal Biology, 2009; 3: 171-174.
12. Paschapur MS, Patil MB, Kumar R, Patil SR. Evaluation of aqueous extract of leaves of Ocimum kilimandscharicum on wound healing activity in albino wistar rats. IJPRIF. 2009; 1(3): 544-50.
13. Dolly G, Nidhi S, Bps S, Shweta R, Shikha A. Ocimum kilimandscharicum: A systematic review. JDDT. 2012; 2(3): 45-52.
14. Samuel lallianrawna, R. Muthukumaran, determination of total phenolic content, total flavanoid content and total antioxidant capacity of ageratinaadenophora(spreng.) king and H .Rob, Sci Vis Vol 13 issue no 4 oct - dec 2013.
15. shweta yadav and padma kumar, production, isolation and identification of flavonoids from aerial parts of hiptagebenghalensis, internatonal journal of life science and pharma research, Vol 2/Issue 3/Jul-Sept 2012.
16. BharathiSambandam, AyyaswamyArivarasan, Extraction and isolation of flavonoid quercetin from the leaves of Trigonellafoenum-graecum and their anti-oxidant activity, International Journal of Pharmacy and Pharmaceutical Sciences, Vol 8, Issue 6, 2016.
17. Mizushima Y and Kobayashi M. Interaction ofanti‐inflammatory drugs with serum preoteins,especially with some biologically active proteins. J of PharmaPharmacol1968; 20:169‐ 173.
18. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences, 2010; 2(1):146-155.
19. Shinde UA, KR Kulkarni, A S Phadke, A M Nair, Dikshit V J Mungantiwar and M N Saraf. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrusdeodara (Roxb.) Loud. Wood Oil. Indian J ExpBiol, 1999; 37(3): 258-261.
20. Azeem AK, Dilip C, Prasanth SS, Junise V, HananShahima. Anti-inflammatory activity of the glandular extracts of Thunnusalalunga. Asia Pac J for Med, 2010; 3(10): 412-20.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © Author(s) retain the copyright of this article.
